The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments† †Electronic supplementary information (ESI) available: Peptide synthesis, full experimental procedures and analytical data of the peptides and products obtained. See DOI: 10.1039/c5sc01415h Click here for additional data file.

Synthesis and stabilities of peptide-based [1]rotaxanes: molecular grafting onto lasso peptide scaffolds† †Electronic supplementary information (ESI) available: Experimental procedures and analytical data of all new compounds. CCDC 1520783. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c7sc00021a Click here for additional data file. Click here for additional data file.

The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments

We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide motif. The resulting peptide conjugates display high DNA affinity and excellent sequence selectivity. Furthermore, the AT-Hook motif also favors the cell internalization of the ...

3-Mercaptopropionic acid-mediated synthesis of peptide and protein thioesters† †Electronic supplementary information (ESI) available: Experimental procedures for the production of 1, all model peptides and proteins and their reactions with MPA including HPLC, LC-MS and NMR characterisation of selected products. See DOI: 10.1039/b815888f Click here for additional data file.

Peptides and proteins fragment sequence-specifically in the presence of 3-mercaptopropionic acid to afford thioesters which can be used in native chemical ligation reactions.

Double quick, double click reversible peptide “stapling”† †Electronic supplementary information (ESI) available: Synthesis and characterization, additional biophysical and biochemical analyses. See DOI: 10.1039/c7sc01342f Click here for additional data file. Click here for additional data file. Click here for additional data file.

Synthesis of S peptides......................................................................................................................... 4 WT-S peptide – 8 ................................................................................................................................ 5 OX-S-Cys peptide – 2c ...................................................................................

Spontaneous head-to-tail cyclization of unprotected linear peptides with the KAHA ligation† †Electronic supplementary information (ESI) available: Experimental procedures and analytical data of all new compounds. See DOI: 10.1039/c5sc01774b Click here for additional data file.